| Description: |
Affinity-purified monoclonal antibody scFv fragments1 (single chain variable fragments) expressed in E.Coli to bind against human cardiac troponin I (cTnI) antigen.
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| Application:
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The fragment contains His and c-Myc fusion tags which can be used for detection or immobilisation.2 The scFv fragment may be suitable for ELISA, western blot, immunohistochemistry, flow cytometry, immunoaffinity purification or other immunological methods.
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| Buffer:
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PBS containing 0.09% sodium azide preservative.*
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| DilnFactor:
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To be defined by end-user
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| Footer:
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* Conjugated scFv is supplied in a separate stabilisation buffer at 4°C
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| Format:
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Affinity purified recombinant antibody fragment with Myc and His detection tags
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| Host:
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E.Coli
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| Immunogen:
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Recombinant human cardiac troponin I (cTnI) antigen.
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| Preservative:
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0.09% Sodium Azide
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| Purity:
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>90% assessed by SDS-PAGE
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| Specificity:
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Tested positive against native human antigen. Shows no cross-reactivity against a panel of unrelated antigens.
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| Storage:
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Frozen at –20°C *
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| References:
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1. Weisser NE, Hall JC. (2009) Applications of single-chain variable fragment antibodies in therapeutics and diagnostics. Biotechnol Adv. 27(4): 502-520. 2. Terpe, K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiol Biotechnol. 60(5):523-33.
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| Datasheet: |
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