Protein Expression Services
Our protein expression services include:
• Bioinformatic analysis and domain selection
• Subclone plasmid / construct expression vectors
• Sequence analysis and verification
• Bacterial cell transformation and clone preparation
• Protein expression and purification
• Optimisation of expression and purification conditions
• Quality control report
• Scale-up production
• Protein characterisation
• Protein lyophilisation - optimsation required - only for large orders
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For over 30 years Randox Life Sciences has developed and manufactured a wide range of products including high quality, cost effective recombinant proteins, polyclonal antibodies and conjugates targeting drugs of abuse, steroids, hormones and biochemical markers. These specific life science products are manufactured at our ISO 13485 certified manufacturing plant in the United Kingdom using state of the art facilities.
Our current product range consists of more than 1000 products including an extensive range of human recombinant proteins specific to a wide range of biochemical markers including Cytokines, Chemokines, Tumour Markers, Adhesion Molecules and Proteases. Our product offering continues to grow in line with customer requirements and Randox’s continuing diagnostic research with more than 200 new products in development.
Our complete range of human recombinant proteins are carrier free and presented in 50% buffered glycerol. All proteins are suitable for long term storage at -20oC for maximum stability. Our recombinant proteins are available in a variety of different sizes generally 10μg, 100μg and 1mg with corresponding antibodies and western blot controls available for the majority of proteins. In addition to this the purity of all proteins is determined by SDS PAGE analysis and quantified using Quantiscan software and guaranteed to be more than 95% pure.
Our comprehensive range of more than 100 recombinant proteins are applicable to the following applications; Western Blot Analysis, Dot Blot Analysis, Competitive and Sandwich ELISA, Immunohistochemistry, Immunoprecipitation, Functionality Testing, Flow Cytometry and Antibody Recognition Assays.
To complement our wide range of ‘off the shelf’ products Randox Life sciences can also provide a broad range of bacterial protein expression services; a complete solution for all your research needs which can be tailored to help you overcome your cloning and protein expression bottleneck. Some of our services include bioinformatic analysis, sequence analysis, bacterial cell transformation, protein expression, purification and characterisation.