| Description: |
Malachite Green has been extensively used for the treatment of fungal infections in fish by the aquaculture industries in Europe, and throughout the world, for many years (7). A recent review has confirmed concerns regarding the use and toxicity of Malachite Green. The toxicity of the dye increases with exposure time, temperature, and concentration and, has been reported to cause carcinogenesis, mutagenesis, chromosomal fractures, teratogenicity, and respiratory toxicity (8). In fish, Malachite Green is extensively metabolized to the colourless compound Leucomalachite Green, which is stored in fat (9). The elimination rate in fish is dependent on the fat content. Residues of Malachite Green and Leucomalachite Green have been reported in serum, liver, kidney, muscle, eggs, and fry (9).
Residue monitoring is reported to be more effective when the sum of Malachite Green and Leucomalachite Green are measured (10). It has been reported that the concentrations of Leucomalachite Green always exceeded those of Malachite Green (11).
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| PH:
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7.2
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| Application:
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ELISA, Western Blot, Dot Blot, Immunohostochemistry, Immunoassays, Development of Rapid tests and other immunoassay,antibody recognition assays
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| Buffer:
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20mM Phosphate, 150mM Sodium Chloride, pH 7.2
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| Format:
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Ig fraction
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| Host:
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Sheep
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| Ig Con:
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8.58mg/ml (U.V. abs @ 280nm)
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| Immunogen:
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Leucomalachite Green-BTG
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| Lot:
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RS476-07/BC4
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| Preservative:
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0.09% Sodium Azide
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| Specificity:
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Compound % Cross-Reactivity
Leucomalachite Green 100
Malachite Green 26.4
Leucocrystal Violet 200
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| Storage:
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≤ -20C, avoid repeated freeze/thaw cycles
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| Work Con:
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0.625μg/ml (EIA)
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| References:
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7. Department of Agriculture and Rural Development, 21st June 2002, 189/02.
8. Srivastava S, Sinha R, Roy D (2004) Toxicological effects of malachite green. Aquat Toxicol., 66(3): 319-29.
9. Mitrowska K, and Posyniak A (2004) Determination of malachite green and its metabolite, leucomalachite green, in fish muscle by liquid chromatography. Bull Vet Inst Pulawy, 48: 173-176.
10. Tarbin JA, Barnes KA, Bygrave J, and Farrington WH (1998) Screening and confirmation of triphenylmethane dyes and their leuco metabolites in trout muscle using HPLC-vis and ESP-LC-MS. Analyst, 123(12): 2567-71.
11. Doerge DR, Churchwell MI, Gehring TA Pu YM, and Plakas SM (1998) Analysis of malachite green and metabolites in fish using liquid chromatography atmospheric pressure chemical ionization mass spectrometry. Rapid Commun Mass Spectrom., 12(21): 1625-34.
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| Datasheet: |
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